Mouse Embryo Assay (MEA), utilizing one and two-cell embryos,
is a reliable and acceptable quality system for detecting toxic
factors within an embryo culture system.
Utilizing FRESHLY HARVESTED one-cell or two-cell mouse embryos,
Embryotech Labs (ELI) offers customized MEA for evaluation of raw
materials, complete culture media, culture media supplements and
various disposable items. Our facility operates under strict GMP
guidelines, which allows us to provide accurate Certificates of
Analysis with confidence. Following is a brief summary of the MEA
offered by ELI.
embryos are harvested from superovulated female B6C3F-1 x B6D2F-1
mice at a standard predetermined hour, post-hCG.
embryos are treated with hyaluronidase and then transferred to
M2 where they are graded and pooled. Two-cell embryos are not
subjected to the enzyme rinse.
standard assay consists of 21 embryos per test item and includes
a 15-embryo control in proven media. Both the test and control
embryos are set up in triplicate under oil and cultured in 5%
CO2 at 37°C until they are scored for development
to expanded blastocysts.
for the one-cell assay is noted at 24 and 96 hours. The two-cell
assay is scored only once at 72 hours.
requires greater than 70% blastocyst formation from the control
group to validate the one-cell assay. Minimum blastocyst
rate is 80% for the two-cell assay.
of Analysis, following strict GMP guidelines, are prepared
and results are reported immediately after final scoring. The
hard copy is faxed or e-mailed and the original
is mailed to your facility.
Testing is performed at ELI Monday through Friday and test items
are received 7 days per week. Because of this flexible schedule,
we offer an immediate turnaround time of 5-7 business days from
test article receipt to reporting results.
Note: The mouse strain, embryo stage and other assay details
are only guidelines and can be modified to suit your particular